Description
Protein Alkaline Phosphatase Labeling Kit - NH2
Alkaline Phosphatase-NH2 Labeling Kit is designed for the simple and rapid preparation of alkaline phosphataselabeled IgG for enzyme immunoassays (EIA) and immunoblotting / immunostaining. It can also be used for the preparation of alkaline phosphatase-labeled antigen for competitive EIA. NH2-reactive Alkaline Phosphatase (AP) (a component of this kit) has activated ester groups and can easily make a covalent bond with an amino group of the target molecule without any activation process. If the target is a small molecule, the conjugate can be purified with the Filtration Tube included in this kit. The Filtration Tube is also used for removing small molecules such as Tris buffer and amine compounds that may interfere with this assay or labeling reaction. This kit contains all of the necessary reagents for alkaline phosphatase labeling, including the storage buffer for the conjugates.